Published online 2017 Oct 12. doi: 10.1038/cddis.2017.514
Manuel Scimeca,1,2,10 Antonietta Salustri,3,10 Elena Bonanno,1,10 Daniela Nardozi,1 Cecilia Rao,4 Eleonora Piccirilli,4 Maurizio Feola,4 Virginia Tancredi,5 Annamaria Rinaldi,5 Giovanni Iolascon,6 Augusto Orlandi,3 Elena Gasbarra,4 Nicola Maffulli,7,8 Maria Luisa Brandi,9 and Umberto Tarantino4,*
Abstract
Pentraxin 3 (PTX3) is a multifunctional glycoprotein regulating inflammatory response, cell proliferation and migration and deposition and remodelling of the extracellular matrix by a variety of cells. In this study, we investigated the possible role of PTX3 in bone homeostasis. To this end, we compared the expression and function of PTX3 in human osteoblasts of osteoporotic, osteoarthritic patients and young subjects not affected by bone diseases. Immunohistochemical analysis performed on bone head biopsies showed a close association between bone health and the number of osteoblasts expressing PTX3. Noteworthy, the proportion of PTX3-positive osteoblasts resulted to be significantly lower in osteoporotic patients compared with both young patients and osteoarthritic patients of the same age. Ex vivo culture of osteoblasts isolated from the three groups of patients confirmed in vivo observation. Specifically, we observed rare runt-related transcription factor 2 (RUNX2) immunopositive osteoblasts expressing PTX3 in cell cultures derived from osteoporotic patients and western blotting analysis showed 80% reduction of PTX3 in the corresponding culture extracts compared with…
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